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Image Search Results
Journal: American Journal of Respiratory and Critical Care Medicine
Article Title: Bronchial Secretory Immunoglobulin A Deficiency Correlates With Airway Inflammation and Progression of Chronic Obstructive Pulmonary Disease
doi: 10.1164/rccm.201010-1629oc
Figure Lengend Snippet: Figure 7. Polymeric immunoglobulin re- ceptor (pIgR) expression and IgA transcytosis in air-liquid interface (ALI) cultured human bronchial epithelial cells (HBECs) grown with or without retinoic acid (RA). (A) Pseu- dostratified ciliated structure of bronchial epithelium and marked secretory compo- nent (SC)/pIgR expression in culture with RA added; squamous stratified structure of bronchial epithelium and absent SC/pIgR expression in culture grown without RA. Top row: hematoxylin and eosin (HE)–stained par- affin sections (original magnification 3400). Bottom row: immunohistochemistry of par- affin tissue sections with goat polyclonal anti–SC-pIgR antibody (original magnifica- tion 3400). (B) Western blot showing ex- pression of SC/pIgR in cell cultures with RA added and no pIgR expression in cells grown without RA. (C) PIGR mRNA expres- sion in RA1 and RA2 cultures (normalized to HPRT). (D) IgA and secretory IgA (SIgA) concentrations in apical washings of ALI cultured HBECs grown with or without RA. * P , 0.001 (compared with RA1 cul- tures). Mean 6 SEM is indicated for each concentration.
Article Snippet:
Techniques: Expressing, Cell Culture, Staining, Immunohistochemistry, Western Blot, Concentration Assay